Model Number: Anti-HCV CLIA01
Brand Name: Egens
Key Specifications/Special Features:
ThisAnti-HCVCLIAisaqualitativemicroplatechemiluminescenceassayforthe invitrodetectionofantibodiestoHepatitisCVirus (HCV)inhumanserumorplasma.Itisintendedforscreeningofblooddonorsand diagnosingpatientsrelatedtoinfectionwithHepatitisCvirus.ASSAY PROCEDUREPrepare Reagents: Dilute 1 volume of Concentrated Washing Buffer (20×) with 19 volumes of distilled water, mix well.Add Samples: Open the foil pouch and remove the Microplate. Set up 1 well as Blank, 2 wells as standard, 1 well as negative control, 1 well as positive control. After dispensing 100μL of Sample Diluent, dispense 25μL of sample or standard or negative control or positive control to the respective wells. Gently vibrating the plate.Incubate: Cover the Microplate with plate cover and incubate the Microplate in a thermostat-controlled water-bath or microplate incubator at 37℃ for 30 minutes.Wash: Wash the Microplate for 5 times. Fill the wells with above 350μL of the diluted Washing Buffer, Soak for above 30 seconds, then aspirate again for each time. Make sure that the rest volume is minimal by tapping plate at absorbent paper.Add Conjugate: Add 100μL of conjugate to each well (except the blank well).Incubate: Cover the Microplate and incubate the plate at 37℃ for 30 minutes.Wash the Plate: Repeat the wash procedure as in step 4.Add Substrate: Add 50μL of Substrate Solution A and 50μL of Substrate Solution B to each well, Vibrate the Microplate. Cover and incubate the Microplate in the dark at room temperature (18-24℃) for 5-10 minutes.Read the RLU value by a microplate chemiluminescence immunoassay analyzer.
Shipping Information:
-
FOB Port:
Shanghai
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Lead Time:
15
-
30
days
Main Export Markets:
- Asia
- Australasia
- Central/South America
- Eastern Europe
- Mid East/Africa
- North America
- Western Europe
By certification [File Integrity]
